Stimulation of polymorphonuclear leukocyte bactericidal activity by supernatants of activated human mononuclear cells

Inhibition of human polymorphonuclear leukocyte respiratory burst, bactericidal activity, and migration by pneumolysin.

The in vitro effects of pneumolysin, a sulfhydryl-activated toxin produced by Streptococcus pneumoniae, on various functions of human polymorphonuclear leukocytes (PMNLs) was investigated. Treatment of PMNLs with highly purified toxin significantly inhibited respiratory burst (in response to stimulation), ability to kill opsonized pneumococci, chemotaxis, and random migration. These inhibitions...

Modulatory activity of chemotherapeutic agents on phagocytosis and intracellular bactericidal activity of human polymorphonuclear and mononuclear phagocytes.

Thirteen chemotherapeutic agents were tested for modulatory activity on phagocytosis by human granulocytes and monocytes. Phagocytosis, phagocytic index, and intracellular bactericidal activity were assessed using Staphylococcus aureus, smooth strain of Escherichia coli, and latex particles. Modulation of phagocytic activity depended on the type of particle used and the presence of serum in the...

Polymorphonuclear Leukocyte Chemotactic Activity

serum and guinea pig serum treated with preformed immune complexes. Normal rabbit sera, guinea pig sera, or rabbit sera deficient in C6 were treated with homologous antibovine serum-albumin-bovine serum-albumin complexes. Sera so treated contained one major PMN chemotactic factor which was heat-stable (56 C for 30 min), had a molecular weight of approximately 15,000, and did not require the pre...

Stimulation of Mononuclear Cells Staphylococcal Enterotoxin C via by Polymorphonuclear Neutrophils Induced Inflammatory Responses of Bovine

The effect of an NADH oxidase inhibitor (hydrocortisone) on polymorphonuclear leukocyte bactericidal activity.

Polymorphonuclear neutrophils (PMN) from patients with chronic granulomatous disease of childhood have impaired bactericidal activity and are deficient in diphosphopyridine nucleotide, reduced form of, (NADH) oxidase. Since hydrocortisone had been shown to inhibit NADH oxidation, experiments were undertaken to determine the effect of hydrocortisone on several parameters of human PMN function. T...